Analyzers Instrumentation

FacScanBD FACScan using BD Cell Quest Pro and Cytek Rainbow software:

This instrument includes two lasers with 488nm and 630nm output wavelengths, with competence for analysis of five colors.It can be used for routine research applications, immunophenotyping, and DNA cell cycle analysis.It is capable of storing 10K cells/sample at 120 samples per hr. using a quartz cuvette flow cell. Only 12x75 polystyrene tubes can be used on this machine.

 

FAS CaliburBD FACScalibur using BD Cell Quest Pro software:

This instrument includes two lasers with 488nm and 640nm output wavelengths with competence for analysis of four colors.It can be used for routine research applications, immunophenotyping, and DNA cell cycle analysis.This machine has selectable flow rates of 12ul/min, 35ul/min, 60ul/min flowing through a quartz cuvette flow cell.Single cell suspensions of 10 to 10 /ml is the recommended range.Only 12x75 polystyrene tubes can be used on this machine.

 

CyAnBeckman Coulter CYAN using Beckman Coulter Summit software:

This instrument includes three lasers with 405nm, 488nm, and 630nm output wavelengths with competence for analysis of nine colors. Applications include immunophenotyping, cell cycle, CFP/YFP and FRET analysis.This machine provides acquisition rates of up to 70,000 events/sec. and analysis capability of up to 10,000,000 event data files.Only 12x75 polypropylene tubes can be used on this machine.

 

 

 

BD LSR II SORP using BD FACSDiva software:

BD LSR II SORP using BD FACSDiva softwareThis instrument includes five lasers with 355nm, 405nm, 488nm, 532nm, and 635nm output wavelengths, with competence for analysis of 18 colors.It can be used for routine research applications, immunophenotyping, DNA cell cycle, calcium flux analysis.Signal processing is powered by the digital acquisition system.Three selectable flow rates of 12ul/min, 35ul/min, 60ul/min with a gain knob providing sample flow rates from 0.5-2x from the midpoint value through a gel coupled quartz cuvette.Maximum data collection of5,000 events/sec.

 

AccuriBD Accuri using CFlow and CFlow Plus software:

This instrument includes two lasers with 488nm and 635nm output wavelengths, with competence for analysis of four colors. Peristaltic pumps provide a non pressurized fluidic system with a six decade range electronic system eliminating the need to adjust detector voltage and gain settings. The machine can run 12x75 tubes or 96 well plates for easy reading of sceening experiments. With CFlow Plus users can define flow rate, core size, and defined stop criteria.

    National Jewish Health Employee Access Only: Online signup done under the National Jewish Health SpyderWeb (internal users only). Click on Cytometry and then new users contact flow lab personnel for registration instructions.

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