Cytometry: Instruments and Services

National Jewish Health
K1014 Goodman Building
303.398.1330 
Fax: 303.398.1225
loomisj@njhealth.org
sobuss@njhealth.org
More info: http://flowlab.njc.org/

Instrument

Main Uses

 

Analyzers

   
LSR
 
II 355nm 405nm 488nm 532nm
635nm lasers
Up to 18 color analysis
CYAN 405nm 488nm 630 lasers Up to 9 color analysis
FACScan 488nm 630nm lasers Up to 5 color analysis
FACScalibur 488nm 640 lasers Up to 4 color analysis
Accuri 488nm 635nm lasers Up to 4 color analysis
IS 100 488nm laser Flow imaging
     

Sorters

   
Synergy 355nm 488nm 640nm
405nm 561nm lasers
Up to12 color sort & analysis
MoFlo XDP 488nm 405nm 635nm lasers Up to 9 color sort & analysis
     

Microscopes

   
IP Lab Long working distance fluorescence
Leica 4 color slides, DIC, Z
LWD Marianas Long working distance, dry objectives
Marianas Multicolor, Z, live cell, Ca++ flux
Olympus Rapid multicolor scope for live cell
Olympus/Apogee Brightfield absorption stained slides
Smith Marianas Inverted multicolor, Z
Zeiss LSM 700 Confocal Multicolor, Z, time, colocalization
MultiPhoton Olympus Deep tissue, in situ live tissue imaging
 

Programs for computational data analysis

AxioVision Zeiss analysis software for widefield system
Cell Quest FCS data on MAC
FloJo FCS on MAC
Ideas Imagestream analysis
Image J NIH image analysis IBM
Iplab Microscope data MAC
LSM 510 Zeiss confocal analysis
NIH Image Microscope data MAC
Real-14 Microscope data IBM
Slidebook Microscope data MAC + IBM
Zen Zeiss confocal analysis
Imaris Microscope data Multiphoton and Zeiss Confocal
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