Method to Produce Monoclonal Antibodies to Human T-Cell Receptor Elements

Tech ID: 91-02

Summary - The specificity of T-cell receptor to an antigen is determined by the five variable elements of the a and ß chains. Research scientists at National Jewish Health have developed a method to produce monoclonal antibodies against any of the five variable elements of human T-cell receptor. This method relies on the transfection of human/mouse hybrid T-cell receptor a or ß chains genes into a mouse T-cell hybridoma lacking an endogenous a or b chain gene.

Potential Applications

  • Research reagents.
  • Monoclonal antibodies for therapeutic use.

Advantages of Invention

  • Simple method
  • Method adaptable to any of the five variable elements of the human T-cell receptor.

State of Development - Scientists have demonstrated that using this method:

  • hVß elements can be expressed on the surface of mouse T-cell hybridomas.
  • The transfected T-cell hybrids are excellent immunogens in mice for hVß.
  • Monoclonal antibodies against hVß13.1 and -13.2 can be raised and be used for the measurements of Vß13.1 and -13.2 in humans.

Licensing Potential - Available for licensing on a non-exclusive basis.

Patent Status - French Patent #0542897.

Inventors - Yongwon Choi, PhD, John Kappler, PhD and Philippa Marrack, PhD

Publication

  • Choi et al. Proc Natl Acad Sci U S A. 1991 Oct 1; 88(19):8357-61.  PMID: 1656442

 

For Further Information, Contact:
Emmanuel Hilaire, PhD
Manager
Technology Transfer Office
National Jewish Health
1400 Jackson Street, Room M206b
Denver, CO 80206
Voice: (303) 398-1262
Fax: (303) 270-2352
HilaireE@njhealth.org

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