C57BL/6 Transgenic Mice Strain Expressing GFP Ubiquitously
Tech ID: 01-05
Research scientists at National Jewish Health have developed an UBI-GFP/BL6 transgenic mouse (C57BL/6 strain) that expresses GFP in all cell types. This characteristic is particularly useful to identify and follow the identification and fate of cells transplanted into another mouse.
- Organ and tissue transplantation
- Tissue development, regeneration and repair
- The UBI-GFP/BL6 transgenic mice are healthy and can be bred to homozygosity.
- The UBI-GFP/BL6 transgenic mice were made directly in the C57BL/6 mouse strain.
- The UBI-GFP/BL6 transgenic mice show remarkably uniform expression of GFP for each cell type studied.
- No loss of GFP fluorescence due to metabolism or partitioning can be detected.
- The GFP gene of these UBI-GFP/BL6 transgenic mice is expressed regardless of tissue type and developmental stage.
State of Development
Scientists have demonstrated by FACS analysis and microscopy that GFP is expressed throughout the mice and that the level of GFP expression is uniform for each cell type studied.
Schaefer et al. 2001 Cellular Immunology214:110.
Brian Schaefer, PhD; Philippa Marrack, PhD, and John Kappler, PhD
Licensing Status - Available for licensing. An additional license from GE Healthcare for the original GFP technology will also be necessary.
For Further Information, Contact:
Emmanuel Hilaire, PhD
Technology Transfer Office
National Jewish Health
1400 Jackson Street, Room M206b
Denver, CO 80206
Voice: (303) 398-1262
Fax: (303) 270-2352