Immune Response Testing & Studies

Advanced Diagnostic Laboratories at National Jewish Health uses a variety of technologies to determine the presence or absence of cell-mediated and antibody-mediated immune responses to drugs, vaccines, toxins and pathogens.

We offer antibody response design, development and validation services to pharmaceutical and biotechnology companies seeking antibody-based measures of efficacy and toxicity for their products, and to laboratories and clinicians seeking antibody-based measures for diagnosis and disease staging.

Advanced Diagnostic Laboratories can utilize human samples and non-human test systems to run your assays.

Immune Cell Function

• ELISpot assays to measure antigen-specific T cell frequency
• Immunophenotype determination by multicolor flow cytometry
• Functional cytotoxicity assays for NK and T cells, including ADCC
• Antigen-specific lymphocyte proliferation assays customized to your needs
• Basophil activation studies (e.g. allergens or drugs)

Antibody Activity

• Antibody responses to new antigens
• Measurement of adverse antibody responses, including IgG, IgM,and IgE

Phospho Flow to Measure Kinase Cascade Activity

Inflammatory Response

• Cytokine measurement: serum, plasma, sputum, and intracellular Toll-like receptor (TLR) functional assays

Complement Activation and Inhibition

• Complement activation products: C3a, C4a, C5a, Bb, SC4b-9 as well as iC3b and C4d In vitro evaluation of the complement activating potential of new compounds, biomaterials of other substances Measurement of autoantibodies against complement components
• Complement functional screens and comprehensive assays for component function and concentration