Isolate Identification
For detailed information about mycobacteriology testing, please visit the Advanced Diagnostic Laboratories Test Menu.
National Jewish Health Mycobacteriology Laboratory receives isolates for identification from several hundred laboratories throughout the United States.
Identification is performed using multiple methods, including genetic probes (MTB complex), DNA sequencing and biochemicals (if needed).
If colony morphology resembles Mycobacterium tuberculosis (MTB) complex, a DNA probe is completed.
If positive, differentiation within the MTB complex and first-line drug antimicrobial susceptibility testing is performed on all initial isolates. Our laboratory will provide a subculture to the respective state public health laboratory for genotyping submission upon request. Please call at 303.398.1339 for more information.
Molecular assays, including rpoB gene and 16S rRNA gene sequencing, are used for identification. Additional distinction within the group Mycobacterium abscessus are made using molecular assays for erm(41) and hsp65 genes.
Members of Mycobacterium tuberculosis (MTB) Complex
Frequently Isolated
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Rarely Isolated
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M. tuberculosis
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M. microti
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M. bovis
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M. caprae
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M. bovis (BCG)
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M. canettii
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M. africanum
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M. pinnipedii
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M. mungi
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